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mouse tlr2 reporter cell line  (InvivoGen)


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    Structured Review

    InvivoGen mouse tlr2 reporter cell line
    EV from mice fed with L. rhamnosus JB-1 reproduce in vitro activity of the original bacterium . (a) <t>TLR2</t> activation by EV was quantified by colorimetric assay using a reporter cell line, with data expressed as a percentage of activity measured for the synthetic TLR2 ligand Pam3CSK4 (300 ng/mL). (b) EV were incubated with BMDCs and subsequent IL-10 expression was measured by flow cytometry. (c) EV were preincubated with T84 cells for 2 h, exposed to 0 or 2.5 ng/mL TNF for 2 h, then IL-8 secretion was measured by ELISA. Error bars represent ± 1 standard error. Each point represents one EV preparation (6–12 mice pooled per preparation). ** p < 0.01, *** p < 0.001
    Mouse Tlr2 Reporter Cell Line, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse tlr2 reporter cell line/product/InvivoGen
    Average 95 stars, based on 96 article reviews
    mouse tlr2 reporter cell line - by Bioz Stars, 2026-02
    95/100 stars

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    1) Product Images from "Bacterial membrane vesicles and phages in blood after consumption of lacticaseibacillus rhamnosus JB-1"

    Article Title: Bacterial membrane vesicles and phages in blood after consumption of lacticaseibacillus rhamnosus JB-1

    Journal: Gut Microbes

    doi: 10.1080/19490976.2021.1993583

    EV from mice fed with L. rhamnosus JB-1 reproduce in vitro activity of the original bacterium . (a) TLR2 activation by EV was quantified by colorimetric assay using a reporter cell line, with data expressed as a percentage of activity measured for the synthetic TLR2 ligand Pam3CSK4 (300 ng/mL). (b) EV were incubated with BMDCs and subsequent IL-10 expression was measured by flow cytometry. (c) EV were preincubated with T84 cells for 2 h, exposed to 0 or 2.5 ng/mL TNF for 2 h, then IL-8 secretion was measured by ELISA. Error bars represent ± 1 standard error. Each point represents one EV preparation (6–12 mice pooled per preparation). ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: EV from mice fed with L. rhamnosus JB-1 reproduce in vitro activity of the original bacterium . (a) TLR2 activation by EV was quantified by colorimetric assay using a reporter cell line, with data expressed as a percentage of activity measured for the synthetic TLR2 ligand Pam3CSK4 (300 ng/mL). (b) EV were incubated with BMDCs and subsequent IL-10 expression was measured by flow cytometry. (c) EV were preincubated with T84 cells for 2 h, exposed to 0 or 2.5 ng/mL TNF for 2 h, then IL-8 secretion was measured by ELISA. Error bars represent ± 1 standard error. Each point represents one EV preparation (6–12 mice pooled per preparation). ** p < 0.01, *** p < 0.001

    Techniques Used: In Vitro, Activity Assay, Activation Assay, Colorimetric Assay, Incubation, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay

    EV isolated from plasma of mice fed L. rhamnosus JB-1 have a distinctive size distribution and contain protein, phage DNA, and lipoteichoic acid of JB-1 origin . (a) Nanoparticle tracking analysis (graph) was used to characterize the size distribution of EV from mice fed JB-1 or PBS vehicle, while transmission electron microscopy (inset images) was used to visualize them. Scale bars represent 50 nm. Ribbon represents ± 1 standard error of 12 EV preparations. Arrow and asterisk indicate region of significant differences ( p < 0.05). (b) Plasma EV from mice fed with CFSE-labeled JB-1 or PBS vehicle were assessed for CFSE-related fluorescence using a plate reader. Data are shown after subtraction of PBS blank wells. (c-e) DNA electrophoresis of qPCR products showing (c) Prophage 1 DNA detected in JB-1 genomic DNA (gDNA) and EV from JB-1-fed mice, but not naïve mouse cecal contents nor EV from PBS-fed mice, whereas (d) Prophage 2 and (e) Prophage 3 were detected only in JB-1 genomic DNA. Full-length gels shown in Fig. S5. (f) EV were assessed for TLR2 activity in a reporter cell line after pre-incubation with or without anti-LTA antibody. Data are expressed as a percentage of activity measured for the synthetic TLR2 ligand Pam3CSK4 (300 ng/mL). (g) BMDCs expressing IL-10 were counted by flow cytometry after pre-incubation with or without anti-LTA antibody and incubation with EV samples. Error bars represent ± 1 standard error. * p < 0.05
    Figure Legend Snippet: EV isolated from plasma of mice fed L. rhamnosus JB-1 have a distinctive size distribution and contain protein, phage DNA, and lipoteichoic acid of JB-1 origin . (a) Nanoparticle tracking analysis (graph) was used to characterize the size distribution of EV from mice fed JB-1 or PBS vehicle, while transmission electron microscopy (inset images) was used to visualize them. Scale bars represent 50 nm. Ribbon represents ± 1 standard error of 12 EV preparations. Arrow and asterisk indicate region of significant differences ( p < 0.05). (b) Plasma EV from mice fed with CFSE-labeled JB-1 or PBS vehicle were assessed for CFSE-related fluorescence using a plate reader. Data are shown after subtraction of PBS blank wells. (c-e) DNA electrophoresis of qPCR products showing (c) Prophage 1 DNA detected in JB-1 genomic DNA (gDNA) and EV from JB-1-fed mice, but not naïve mouse cecal contents nor EV from PBS-fed mice, whereas (d) Prophage 2 and (e) Prophage 3 were detected only in JB-1 genomic DNA. Full-length gels shown in Fig. S5. (f) EV were assessed for TLR2 activity in a reporter cell line after pre-incubation with or without anti-LTA antibody. Data are expressed as a percentage of activity measured for the synthetic TLR2 ligand Pam3CSK4 (300 ng/mL). (g) BMDCs expressing IL-10 were counted by flow cytometry after pre-incubation with or without anti-LTA antibody and incubation with EV samples. Error bars represent ± 1 standard error. * p < 0.05

    Techniques Used: Isolation, Transmission Assay, Electron Microscopy, Labeling, Fluorescence, Nucleic Acid Electrophoresis, Activity Assay, Incubation, Expressing, Flow Cytometry

    Estimation of bacterial MV in plasma EV isolated from mice fed L. rhamnosus JB-1 . We enumerated JB-1 MV by nanoparticle tracking analysis and used these to produce standard curves for (a) MV activation of TLR2 in a reporter cell line, (b) CFSE fluorescence after labeling MV with CFSE, and (c) qPCR cycle number at which Prophage 1 DNA was detected (ct). Horizontal dotted lines show the average values of EV from JB-1-fed mice in the same assays. Where the horizontal line and trend of response intersect is an estimate of the number of MV in an average EV preparation
    Figure Legend Snippet: Estimation of bacterial MV in plasma EV isolated from mice fed L. rhamnosus JB-1 . We enumerated JB-1 MV by nanoparticle tracking analysis and used these to produce standard curves for (a) MV activation of TLR2 in a reporter cell line, (b) CFSE fluorescence after labeling MV with CFSE, and (c) qPCR cycle number at which Prophage 1 DNA was detected (ct). Horizontal dotted lines show the average values of EV from JB-1-fed mice in the same assays. Where the horizontal line and trend of response intersect is an estimate of the number of MV in an average EV preparation

    Techniques Used: Isolation, Activation Assay, Fluorescence, Labeling



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    EV from mice fed with L. rhamnosus JB-1 reproduce in vitro activity of the original bacterium . (a) <t>TLR2</t> activation by EV was quantified by colorimetric assay using a reporter cell line, with data expressed as a percentage of activity measured for the synthetic TLR2 ligand Pam3CSK4 (300 ng/mL). (b) EV were incubated with BMDCs and subsequent IL-10 expression was measured by flow cytometry. (c) EV were preincubated with T84 cells for 2 h, exposed to 0 or 2.5 ng/mL TNF for 2 h, then IL-8 secretion was measured by ELISA. Error bars represent ± 1 standard error. Each point represents one EV preparation (6–12 mice pooled per preparation). ** p < 0.01, *** p < 0.001
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    EV from mice fed with L. rhamnosus JB-1 reproduce in vitro activity of the original bacterium . (a) <t>TLR2</t> activation by EV was quantified by colorimetric assay using a reporter cell line, with data expressed as a percentage of activity measured for the synthetic TLR2 ligand Pam3CSK4 (300 ng/mL). (b) EV were incubated with BMDCs and subsequent IL-10 expression was measured by flow cytometry. (c) EV were preincubated with T84 cells for 2 h, exposed to 0 or 2.5 ng/mL TNF for 2 h, then IL-8 secretion was measured by ELISA. Error bars represent ± 1 standard error. Each point represents one EV preparation (6–12 mice pooled per preparation). ** p < 0.01, *** p < 0.001
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    EV from mice fed with L. rhamnosus JB-1 reproduce in vitro activity of the original bacterium . (a) <t>TLR2</t> activation by EV was quantified by colorimetric assay using a reporter cell line, with data expressed as a percentage of activity measured for the synthetic TLR2 ligand Pam3CSK4 (300 ng/mL). (b) EV were incubated with BMDCs and subsequent IL-10 expression was measured by flow cytometry. (c) EV were preincubated with T84 cells for 2 h, exposed to 0 or 2.5 ng/mL TNF for 2 h, then IL-8 secretion was measured by ELISA. Error bars represent ± 1 standard error. Each point represents one EV preparation (6–12 mice pooled per preparation). ** p < 0.01, *** p < 0.001
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    Image Search Results


    EV from mice fed with L. rhamnosus JB-1 reproduce in vitro activity of the original bacterium . (a) TLR2 activation by EV was quantified by colorimetric assay using a reporter cell line, with data expressed as a percentage of activity measured for the synthetic TLR2 ligand Pam3CSK4 (300 ng/mL). (b) EV were incubated with BMDCs and subsequent IL-10 expression was measured by flow cytometry. (c) EV were preincubated with T84 cells for 2 h, exposed to 0 or 2.5 ng/mL TNF for 2 h, then IL-8 secretion was measured by ELISA. Error bars represent ± 1 standard error. Each point represents one EV preparation (6–12 mice pooled per preparation). ** p < 0.01, *** p < 0.001

    Journal: Gut Microbes

    Article Title: Bacterial membrane vesicles and phages in blood after consumption of lacticaseibacillus rhamnosus JB-1

    doi: 10.1080/19490976.2021.1993583

    Figure Lengend Snippet: EV from mice fed with L. rhamnosus JB-1 reproduce in vitro activity of the original bacterium . (a) TLR2 activation by EV was quantified by colorimetric assay using a reporter cell line, with data expressed as a percentage of activity measured for the synthetic TLR2 ligand Pam3CSK4 (300 ng/mL). (b) EV were incubated with BMDCs and subsequent IL-10 expression was measured by flow cytometry. (c) EV were preincubated with T84 cells for 2 h, exposed to 0 or 2.5 ng/mL TNF for 2 h, then IL-8 secretion was measured by ELISA. Error bars represent ± 1 standard error. Each point represents one EV preparation (6–12 mice pooled per preparation). ** p < 0.01, *** p < 0.001

    Article Snippet: TLR2 ligand presence was determined using a mouse TLR2 reporter cell line (HEK-Blue-mTLR2; Invivogen) following manufacturer’s directions and as recently described.

    Techniques: In Vitro, Activity Assay, Activation Assay, Colorimetric Assay, Incubation, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay

    EV isolated from plasma of mice fed L. rhamnosus JB-1 have a distinctive size distribution and contain protein, phage DNA, and lipoteichoic acid of JB-1 origin . (a) Nanoparticle tracking analysis (graph) was used to characterize the size distribution of EV from mice fed JB-1 or PBS vehicle, while transmission electron microscopy (inset images) was used to visualize them. Scale bars represent 50 nm. Ribbon represents ± 1 standard error of 12 EV preparations. Arrow and asterisk indicate region of significant differences ( p < 0.05). (b) Plasma EV from mice fed with CFSE-labeled JB-1 or PBS vehicle were assessed for CFSE-related fluorescence using a plate reader. Data are shown after subtraction of PBS blank wells. (c-e) DNA electrophoresis of qPCR products showing (c) Prophage 1 DNA detected in JB-1 genomic DNA (gDNA) and EV from JB-1-fed mice, but not naïve mouse cecal contents nor EV from PBS-fed mice, whereas (d) Prophage 2 and (e) Prophage 3 were detected only in JB-1 genomic DNA. Full-length gels shown in Fig. S5. (f) EV were assessed for TLR2 activity in a reporter cell line after pre-incubation with or without anti-LTA antibody. Data are expressed as a percentage of activity measured for the synthetic TLR2 ligand Pam3CSK4 (300 ng/mL). (g) BMDCs expressing IL-10 were counted by flow cytometry after pre-incubation with or without anti-LTA antibody and incubation with EV samples. Error bars represent ± 1 standard error. * p < 0.05

    Journal: Gut Microbes

    Article Title: Bacterial membrane vesicles and phages in blood after consumption of lacticaseibacillus rhamnosus JB-1

    doi: 10.1080/19490976.2021.1993583

    Figure Lengend Snippet: EV isolated from plasma of mice fed L. rhamnosus JB-1 have a distinctive size distribution and contain protein, phage DNA, and lipoteichoic acid of JB-1 origin . (a) Nanoparticle tracking analysis (graph) was used to characterize the size distribution of EV from mice fed JB-1 or PBS vehicle, while transmission electron microscopy (inset images) was used to visualize them. Scale bars represent 50 nm. Ribbon represents ± 1 standard error of 12 EV preparations. Arrow and asterisk indicate region of significant differences ( p < 0.05). (b) Plasma EV from mice fed with CFSE-labeled JB-1 or PBS vehicle were assessed for CFSE-related fluorescence using a plate reader. Data are shown after subtraction of PBS blank wells. (c-e) DNA electrophoresis of qPCR products showing (c) Prophage 1 DNA detected in JB-1 genomic DNA (gDNA) and EV from JB-1-fed mice, but not naïve mouse cecal contents nor EV from PBS-fed mice, whereas (d) Prophage 2 and (e) Prophage 3 were detected only in JB-1 genomic DNA. Full-length gels shown in Fig. S5. (f) EV were assessed for TLR2 activity in a reporter cell line after pre-incubation with or without anti-LTA antibody. Data are expressed as a percentage of activity measured for the synthetic TLR2 ligand Pam3CSK4 (300 ng/mL). (g) BMDCs expressing IL-10 were counted by flow cytometry after pre-incubation with or without anti-LTA antibody and incubation with EV samples. Error bars represent ± 1 standard error. * p < 0.05

    Article Snippet: TLR2 ligand presence was determined using a mouse TLR2 reporter cell line (HEK-Blue-mTLR2; Invivogen) following manufacturer’s directions and as recently described.

    Techniques: Isolation, Transmission Assay, Electron Microscopy, Labeling, Fluorescence, Nucleic Acid Electrophoresis, Activity Assay, Incubation, Expressing, Flow Cytometry

    Estimation of bacterial MV in plasma EV isolated from mice fed L. rhamnosus JB-1 . We enumerated JB-1 MV by nanoparticle tracking analysis and used these to produce standard curves for (a) MV activation of TLR2 in a reporter cell line, (b) CFSE fluorescence after labeling MV with CFSE, and (c) qPCR cycle number at which Prophage 1 DNA was detected (ct). Horizontal dotted lines show the average values of EV from JB-1-fed mice in the same assays. Where the horizontal line and trend of response intersect is an estimate of the number of MV in an average EV preparation

    Journal: Gut Microbes

    Article Title: Bacterial membrane vesicles and phages in blood after consumption of lacticaseibacillus rhamnosus JB-1

    doi: 10.1080/19490976.2021.1993583

    Figure Lengend Snippet: Estimation of bacterial MV in plasma EV isolated from mice fed L. rhamnosus JB-1 . We enumerated JB-1 MV by nanoparticle tracking analysis and used these to produce standard curves for (a) MV activation of TLR2 in a reporter cell line, (b) CFSE fluorescence after labeling MV with CFSE, and (c) qPCR cycle number at which Prophage 1 DNA was detected (ct). Horizontal dotted lines show the average values of EV from JB-1-fed mice in the same assays. Where the horizontal line and trend of response intersect is an estimate of the number of MV in an average EV preparation

    Article Snippet: TLR2 ligand presence was determined using a mouse TLR2 reporter cell line (HEK-Blue-mTLR2; Invivogen) following manufacturer’s directions and as recently described.

    Techniques: Isolation, Activation Assay, Fluorescence, Labeling